ISSN 1308-8734 | E-ISSN 1308-8742
Original Article
The Effects of Amlodipine on the Biochemical and Histopathological Changes in the Rabbit Ileum Subjected to Ischemia-Reperfusion
1 Department of General Surgery, Gulhane Military Medical Academy, Ankara, Turkey  
2 Department of Pathology, Gulhane Military Medical Academy, Ankara, Turkey  
3 Department of Pharmacology, Gulhane Military Medical Academy, Ankara, Turkey  
4 Department of Pediatric surgery, Gulhane Military Medical Academy, Ankara, Turkey  
5 Department of Pharmacology, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey  
6 Department of Anatomy, Gulhane Military Medical Academy, Ankara, Turkey  
Eurasian J Med 2011; 43: 33-38
DOI: 10.5152/eajm.2011.07
Key Words: Amlodipine, Ischemia-Reperfusion, Glutathione, Superoxide Dismutase, Malonyldialdehyde, Rabbit
Abstract

 

Objective: The aim of this study was to determine the potential, protective effects of amlodipine in an experimental, ischemia-reperfusion (I/R) model in the rabbit small intestine.

Materials and Methods: The rabbits were divided into four groups: sham-operated, amlodipine (10 mg/kg) + sham-operated, I/R, and I/R + amlodipine (10 mg/kg) groups. An intestinal I/R model was applied to the rabbits. The superior mesenteric artery was occluded for 1 h with an atraumatic vascular clamp and then was reperfused for 2 h. Animals in the amlodipine and I/R + amlodipine groups received the amlodipine by oral gavage. At the end of the 2-h-reperfusion period, the animals were sacrificed.

Results: Pretreatment with amlodipine significantly increased SOD activity and GSH levels to values close to those found in the serum from the I/R group. Rabbits in the I/R group showed high levels of serum MDA. Amlodipine pretreatment significantly reduced the serum MDA levels compared to the I/R group, although the MDA levels in the I/R + amlodipine group were still higher than in the sham-operated group. The I/R damage was ameliorated by amlodipine pretreatment, as evidenced by histopathological analysis.

Conclusion: The present study is the first to report an attenuation of I/R-induced intestinal injury by the systemic administration of amlodipine.

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