ISSN 1308-8734 | E-ISSN 1308-8742
Original Article
The Effects of Erdosteine and N-Acetylcysteine on Apoptotic and Antiapoptotic Markers in Pulmonary Epithelial Cells in Sepsis
1 Department of Pulmonary Medicine, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey  
2 Department of Pathology, Faculty of Medicine, Atatürk University, Erzurum, Turkey  
Eurasian J Med 2013; 45: 167-175
DOI: 10.5152/eajm.2013.35
Key Words: Apoptosis, epithelial cells, erdosteine, N-acetylcysteine, pathogenesis, sepsis

 Objective: This study investigated the frequency of apoptosis in rat pulmonary epithelial cells after the injection of an intraperitoneal endotoxin lipopolysaccharide (LPS), the effects of LPS on apoptotic (bax, caspase-3) and antiapoptotic (bcl-2) markers during lung damage, and the protective effects of two known antioxidant agents, erdosteine and N-acetylcysteine (NAC).


Materials and Methods: Male Wistar rats were divided into the following six groups, which included nine rats each: two control groups, two LPS-treated groups, one erdosteine-treated group (150 mg/kg), and one NAC-treated group (150 mg/kg). LPS was injected intraperitoneally at a dosage of 20 mg/kg. Following LPS injection, the antioxidants were orally administered. The rats were sacrificed at 24 h after LPS administration. The levels of apoptosis in bronchiolar and alveolar cells were determined using the TUNEL-staining method. Immunohistochemical staining of cytoplasmic bax, caspase-3, and bcl-2 in the epithelial cells was performed.


Results: Erdosteine and NAC significantly reduced the rate of LPS-induced pulmonary epithelial cell apoptosis. The effect of NAC on regulating apoptosis was weaker than that of erdosteine. Erdosteine and NAC significantly reduced the local induction of bax and caspase 3 and significantly increased the reduced local production of bcl-2.


Conclusion: These findings suggest that erdosteine and NΑC can effectively protect the lungs from the damaging effects of LPS.  

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