ISSN 1308-8734 | E-ISSN 1308-8742
Original Article
XRCC1 Gene Polymorphisms and miR-21 Expression in Patients with Colorectal Carcinoma
1 Department of Biochemistry, Cairo University School of Medicine, Cairo, Egypt; Department of Pharmacology, Hail University College of Pharmacy, Hail, Saudi Arabia  
2 Department of Biochemistry, Cairo University School of Medicine, Cairo, Egypt.  
3 Department of Tropical Medicine and Gastroenterology, Cairo University School of Medicine, Cairo, Egypt  
Eurasian J Med 2017; 49: 132-136
DOI: 10.5152/eurasianjmed.2017.17021
Key Words: Colorectal cancer, miRNA-21, XRCC1, DNA repair, single nucleotide polymorphisms
Abstract

Objective: The objectives of this study were to evaluate the impact of two X-ray repair cross complementing 1 (XRCC1) gene polymorphisms (Arg194Trp and Arg399Gln) on the risk of development of colorectal cancer (CRC) and to assess the expression levels of microRNA-21 (miR-21) in CRC patients.

 

Materials and Methods: A case-control cross sectional study was conducted on 50 CRC patients and 50 cancer-free subjects. DNA and miR-21 were extracted from whole blood samples. The expression levels of the XRCC1 polymorphisms and miR-21 were assessed by real-time PCR in all subjects of the study.

 

Results: Genotype analysis revealed a significant association between CRC risk and both the Arg194Trp genotype (OR=11.407, 95% CI=4.039-32.221, p<0.001) and the Arg399Gln genotype (OR=3.778, 95% CI= 1.6-8.919, p=0.002). The expression levels of circulating miR-21 were able to detect CRC cases significantly (p=0.022) with a sensitivity of 82% and a specificity of 56% (Area under the curve (AUC)=0.633) but were unable to distinguish between early and late cases (AJCC classification) (p=0.194).

 

 

Conclusion: The XRCC1 Arg194Trp and Arg399Gln polymorphisms both confer high susceptibility for the development of CRC. Circulating miR-21 expression levels are a potentially diagnostic non-invasive genetic marker of CRC.

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