ISSN 1308-8734 | E-ISSN 1308-8742
Original Article
Effect of Nigella sativa Ethanol Extract on the Nitric Oxide Content and Renal Arteriole Diameter of a Pre-eclampsia Mouse Model
1 Midwifery Magister Program, Brawijaya University, Malang, East Java and Polytechnic of Health Denpasar, Bali, Indonesia  
2 Department of Obstetrics and Gynecology, Dr. Saiful Anwar Regional Public Hospital, Malang, East Java, Indonesia  
3 Laboratory of Parasitology, Brawijaya University School of Medicine, Malang, East Java, Indonesia  
Eurasian J Med 2018; 50: 148-151
DOI: 10.5152/eurasianjmed.2018.17123
Key Words: Nitric oxide, Nigella sativa, pre-eclampsia, renal arteriole diameter
Abstract

Objective: The aim of this study was to investigate the effect of Nigella sativa ethanol extract on nitric oxide (NO) levels and renal arteriole diameter of a pre-eclampsia mouse model.


Materials and Methods
: This experimental study was conducted using a post-test only control group design. Thirty BALB/c mice were divided into six groups: one negative control (normal pregnant mice), one positive control (pre-eclampsia model), and four groups of pre-eclampsia mice treated with varying doses of N. sativa (500 mg/kg body weight (BW)/day, 1000 mg/kg BW/day, 1500 mg/kg BW/day, and 2000 mg/kg BW/day). Ethanol extract of N. sativa was given for 5 days. Data were analyzed using analysis of variance.


Results
: We detected significant differences in NO levels between the pre-eclampsia mouse model and those given the ethanol extract. The latter had NO levels of 85.77±4.47 µM (500 mg/kg BW/day), 189.04±6.01 µM (1000 mg/kg BW/day), 226.56±2.13 µM (1500 mg/BW/day), and 207.98±4.74 µM (2000 mg/kg BW/day). The mean renal arteriole diameter showed significant differences among the treatment groups with N. sativa doses of 1000, 1500, and 2000 mg (15.15±2.21b μm, 16.35±2.52b μm, and 15.76±3.03b µm, respectively).


Conclusion
: N. sativa ethanol extract treatment increases NO levels and enlarges renal arteriole diameter of a pre-eclampsia mouse model in a dose-dependent manner.


Cite this article as
: Purnamayanti NMD, Windu SC, Poeranto S. Effect of Nigella sativa Ethanol Extract on the Nitric Oxide Content and Renal Arteriole Diameter of a Pre-eclampsia Mouse Model. Eurasian J Med 2018; 50(3): 148-151.

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