The Effects of Amlodipine on the Biochemical and Histopathological Changes in the Rabbit Ileum Subjected to Ischemia-Reperfusion
Department of General Surgery, Gulhane Military Medical Academy, Ankara, Turkey
Department of Pathology, Gulhane Military Medical Academy, Ankara, Turkey
Department of Pharmacology, Gulhane Military Medical Academy, Ankara, Turkey
Department of Pediatric surgery, Gulhane Military Medical Academy, Ankara, Turkey
Department of Pharmacology, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey
Department of Anatomy, Gulhane Military Medical Academy, Ankara, Turkey
EAJM 2011; 43: 33-38
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Key Words: Amlodipine, Ischemia-Reperfusion, Glutathione, Superoxide Dismutase, Malonyldialdehyde, Rabbit
Objective: The aim of this study was to determine the potential, protective effects of amlodipine in an experimental, ischemia-reperfusion (I/R) model in the rabbit small intestine.
Materials and Methods: The rabbits were divided into four groups: sham-operated, amlodipine (10 mg/kg) + sham-operated, I/R, and I/R + amlodipine (10 mg/kg) groups. An intestinal I/R model was applied to the rabbits. The superior mesenteric artery was occluded for 1 h with an atraumatic vascular clamp and then was reperfused for 2 h. Animals in the amlodipine and I/R + amlodipine groups received the amlodipine by oral gavage. At the end of the 2-h-reperfusion period, the animals were sacrificed.
Results: Pretreatment with amlodipine significantly increased SOD activity and GSH levels to values close to those found in the serum from the I/R group. Rabbits in the I/R group showed high levels of serum MDA. Amlodipine pretreatment significantly reduced the serum MDA levels compared to the I/R group, although the MDA levels in the I/R + amlodipine group were still higher than in the sham-operated group. The I/R damage was ameliorated by amlodipine pretreatment, as evidenced by histopathological analysis.
Conclusion: The present study is the first to report an attenuation of I/R-induced intestinal injury by the systemic administration of amlodipine.