The Eurasian Journal of Medicine
Original Article
Protective Effect of Udenafil Against Ischemia Reperfusion Injury Due to Testicular Torsion/Detorsion in Rat Model

Protective Effect of Udenafil Against Ischemia Reperfusion Injury Due to Testicular Torsion/Detorsion in Rat Model

1.

Department of Urology, Memorial Hospital, Diyarbakir, Turkey

2.

Department of Urology, Medistate Hospital, Istanbul, Turkey

3.

Department of Urology, Haydarpasa Numune Training and Research Hospital, Istanbul, Turkey

4.

Department of Pathology, Haydarpasa Numune Training and Research Hospital, Istanbul, Turkey

5.

Department of Urology, Fatih Sultan Mehmet Training and Research Hospital, Istanbul, Turkey

Eurasian J Med 2020; 52: 115-119
DOI: 10.5152/eurasianjmed.2020.19229
Read: 33 Downloads: 14 Published: 05 June 2020

Objective: Testicular torsion causes migration of neutrophils to the ischemic region and formation of free oxygen radicals that have a critical effect on ischemic reperfusion (I/R) injury. Udenafil is a selective, strong, and reversible inhibitor of phosphodiesterase type enzyme. In our study, we evaluate the protective effect of udenafil against reperfusion injury due to I/R.

Materials and Methods: Twenty-one male, adult, Wistar-Albino rats aged 8 months were randomly divided into three groups; sham, I/R, and I/R+udenafil. One hour before the detorsion operation, the sham and I/R groupssaline, and I/R+udenafil groups were administered 2 mg/kg udenafil intraperitoneally. Blood samples were collected to evaluate the inflammatory mediators. Spermatogenic factors were evaluated according to Johnsen criteria.

Results: Histopathological and molecular parameters from all groups were compared. Mean values of TNF-α and IL-1β in venous blood samples were calculated. We observed that TNF-a values were statistically significantly increased in the I/R group than those in sham groups, and these values were decreased with udenafil treatment Furthermore, the glutathione peroxidase (GPx) level was statistically significantly decreased in the I/R group, and treatment with udenafil prevented this decrease. Evaluation of spermatogenesis using the Johnsen scoring system showed no statistically significant difference in mean scores between the groups.

Conclusion: We concluded that deterioration of biochemical and histopathological parameters are reversed, and injury due to I/R in testicle tissue may be decreased with udenafil treatment. Results of this experimental study show that efficacy of the udenafil treatment in testis torsion should be investigated.

Cite this article as: Sertkaya Z, Koca O, Ozturk M, et al.  Protective Effect of Udenafil Against Ischemia Reperfusion İnjury Due to Testicular Torsion/Detorsion in Rat Model. Eurasian J Med 2020; 52(2): 115-9.

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